| Ruth Reynolds, Nutritionist in Brighton | Nutritionist | 1 Hillcrest | Mon–Fri 09:00–15:00 |
| Sackville Construction Ltd | Construction company | 52 Bristol Gardens | Mon–Fri 07:00–18:00 |
| Safe Haven Sussex | Homeless service | 191 Freshfield Rd | Mon–Thu 09:00–17:00 |
| Salsa Zone | Dance school | Vk Revolution, 77 West St | Mon–Thu 08:30–20:00 |
| Salt & Charcoal | Greek restaurant | 57 London Rd | Daily 11:00–00:00 |
| Salt Shed | Meat dish restaurant | 111 Church St | Mon–Thu 17:00–22:00 |
| Salvage & Sawdust Co | Vintage clothing shop | 36 Meeting House Ln | Mon–Thu 10:00–17:00 |
| Sapphire Goldsmith & Workshop | Jewellery store | 41a Brighton Square | Mon–Fri 10:00–17:00 |
| School of Architecture, Technology and Engineering | University department | Mithras House, Lewes Rd | Mon–Fri 08:00–19:00 |
| Se Surveying Ltd | Land surveyor | 70 Ladies' Mile Rd | Mon–Fri 09:00–17:00 |
| Seaside Splatter | Art studio | 49 Norfolk Square | Mon–Fri 13:50–19:20 |
| Sensor House | Business centre | 10-12 Lewes Rd | Mon–Fri Open 24 hours |
| Seven Cellars @ Brighton Station | Off licence | Unit 6, Brighton Railway Station | Thu 10:00–20:00 |
| Sew Fabulous CIC | Education centre | Studio 10, Marshalls Row | Sat 10:00–18:00 |
| Shaanxi 一面如旧 | Chinese noodle restaurant | 48 Preston St | Thu 12:00–14:30, 17:00–21:30 |
| Shabitat | Second-hand furniture shop | Saunders Park Depot, Lewes Rd | Mon–Sat 10:30–17:00 |
| Sharon Collins Resource Centre | Adult day care center | Sharon Collins Resource Centre, 13 Albion St | Mon–Fri 08:30–16:30 |
| She Said | Lingerie shop | 12a Ship St Gardens | Mon 13:00–17:30 |
| Shear Architectural Design Ltd | Architectural designer | Maritime House, Basin Rd N | Mon–Fri 09:00–17:30 |
| Shimaguni Japanese Lessons | Language school | 92B Trafalgar St | Mon–Fri 09:00–21:00 |
| Shine hair extensions | Hair extension technician | Highlands Rd | Tue–Fri 10:00–16:00 |
| Sideline Screenprinting | Printing shop | Rodhus, 16-30 Hollingdean Rd | Mon–Fri 09:00–17:00 |
| Siesta Crafts | Arts and crafts shop | 26A Trafalgar St | Mon–Sat 10:30–18:00 |
| Simply Business Finance Ltd | Finance broker | The Offices, 57 Newtown Rd | Mon–Thu 09:00–17:30 |
| Singing Lessons And Trans Voice Alteration / Gender Affirming Voice Coaching | Vocal instructor | Under The Bridge Studios, 7 Trafalgar Arches | Tue–Wed 10:00–19:00 |
| SkyHook Storage & Removals | Storage facility | Boundary Rd | Mon–Fri 06:00–19:00 |
| Skyreach Solutions | Aerial photographer | 2 Norwich Cl | Daily Open 24 hours |
| Sleep Through Jen | Sleep clinic | 6 Park Crescent Pl | Mon Open 24 hours |
| Small Street Escooters | Scooter repair shop | Behind 'Magic Man, 17 Gordon Rd | Thu–Fri 10:00–18:00 |
| smart of Brighton | Smart car dealer | Victoria Rd | Mon–Fri 08:00–18:00 |