| A A Taylor | Joiner | 11-13 Shaftesbury Pl | Mon–Fri 08:00–17:30 |
| A Coombs Pet Centre (Brighton) | Pet care store | 19-21 Baker St | Thu 08:30–17:45 |
| A R Kirk Clockmaker | Clock repair service | 42a Robertson Rd | Mon 09:00–14:30 |
| A Shade Above | Lamp shade supplier | Regency House, North St | Mon–Fri 09:00–17:00 |
| A1 Glass & Glazing - Double Glazing Brighton | Glass repair service | Unit 11, Bell Tower Industrial Estate, Roedean Rd | Thu 08:00–16:00 |
| AA Mobile Tyre | Tyre manufacturer | 4 Bodiam Cl | Thu 07:00–23:00 |
| AB Sports Nutrition | Food manufacturer | Sea View Way | Mon–Thu 09:00–17:00 |
| AC South | Air conditioning contractor | Portland Road Trading Estate, Unit 8 Victoria, Victoria Rd | Mon–Fri 08:30–17:00 |
| Adelfia Brighton | Greek restaurant | 13-14 Preston St | Thu 17:00–22:00 |
| Adept Occupational Health Ltd | Occupational health service | 83 Queens Rd | Mon–Fri 09:00–17:00 |
| Adetiq Ltd | Business-to-business service | Avery House, 69 North St | Mon–Fri 08:30–17:00 |
| Adult Skills Hub | Adult education centre | Jubilee Library | Mon–Fri 10:00–17:00 |
| Aerials and More | Satellite communication service | 15 Westfield Cl | Fri 09:00–18:00 |
| Afrori Books | Book shop | Brighton and Hove, Brighton BN1 1YD | Sat 10:30–18:00 |
| Airsports Sussex | Flight school | 332 Mile Oak Rd | Daily 09:00–17:00 |
| Al Tawfeeq Transport | Transportation service | 25A Park Crescent Terr | Daily Open 24 hours |
| ALBA Driving School Brighton | Driving school | 32 Wilmington Way | Mon–Fri 07:00–20:00 |
| Albion Inventory | Property management company | Crest Way | Mon–Fri 09:00–17:00 |
| Albion Window Cleaning Ltd | Window cleaning service | Unit 4 Freemans Road Southern Cross Workshops | Mon 07:00–17:00 |
| Alchymis | Herbalist | Unit 3, Bank Chambers, Preston Circus | Tue–Fri 10:00–18:00 |
| Alderson & Co Quantity Surveyors & RICS Home Surveys Brighton & Hove | Property surveyor | 61 Boundary Rd | Mon–Fri 08:30–17:00 |
| Ali Gas Service Limited | Gas shop | 9 Langley Cres | Daily Open 24 hours |
| Alice Eden Jewellery | Jewellery designer | 23 Crescent Rd | Mon 09:00–18:00 |
| Alison Sutcliffe Counselling, Psychotherapy, EMDR & Clinical Supervision | Psychotherapist | 30 Grand Parade | Mon–Thu 09:00–16:00 |
| Allsorts Youth Project | Youth organisation | 69 Ship St | Mon–Fri 09:00–17:00 |
| Alternative motorcycle training ltd | Motorcycle driving school | Woodingdean Primary School, Warren Rd | Daily 08:30–17:00 |
| Andrew Beaton Music | Music producer | 5 Buckingham Pl | Mon–Fri 08:30–19:30 |
| Anthony Meadows Antique furniture restoration. | Antique furniture restoration service | 11 Court Cl | Mon–Fri 08:00–17:30 |
| April March Jewellery | Goldsmith | 35A Vale Rd | Mon–Fri 09:00–17:00 |
| Arcadia Cigars - Bond Street | Cigar shop | 6 Bond St | Mon–Fri 10:00–18:00 |