| Archaeology South-East | Heritage preservation | 2 Chapel Pl | Mon–Fri 08:00–17:00 |
| Armed Forces Careers Office - Brighton | Military recruiting office | 120-121 Queens Rd | Mon–Fri 09:00–17:30 |
| Arthur Denyer Funeral Directors | Funeral home | 18-19 Lewes Rd | Daily Open 24 hours |
| Artistic MetalWorks | Steel fabricator | Unit 1 Freemans Rd | Mon–Fri 08:30–17:00 |
| Aspen Brodie Physiotherapy | Physiotherapist | 111 Cres Dr N | Mon–Wed 09:00–17:00 |
| Atom Graphics Animation | Animation studio | Fl 5 & 6, Tower Point, 44 North Rd | Mon–Fri 09:00–18:00 |
| atomos | Investment service | Ground Floor North, 126 Dyke Rd | Mon–Fri 09:00–17:00 |
| Attuned Therapy | EMDR psychotherapist | 22 Hollingbury Rd | Mon 10:00–18:30 |
| Autovets Brighton - German, and Sports Car Specialists | Mechanic | 536-540 Falmer Rd | Mon–Fri 08:00–17:00 |
| Ayurveda Brighton | Ayurvedic clinic | 123 Cuckmere Way | Mon–Tue 09:00–17:00 |
| Ayuseva | Alternative medicine practitioner | 23 Green Ridge | Mon–Fri 09:00–17:00 |
| B Company 3PWRR | Military base | 198 Dyke Rd | Mon–Tue 09:00–17:00 |
| Baby Bao | Taiwanese restaurant | The Pond, 49 Gloucester Rd | Mon–Fri 12:00–15:00, 17:00–21:00 |
| Badger Clothing Co Ltd | Men's clothes shop | 26 Bond St | Thu 09:30–18:00 |
| Badgers Tennis Club | Tennis club | Church Pl | Mon–Fri 09:00–18:00 |
| Bagelman | Bagel shop | 7 Bond St | Mon–Fri 09:00–16:00 |
| Bakers, Co-op Funeralcare | Funeral home | 60 Church Rd | Daily Open 24 hours |
| Balmonds Skincare Ltd | Cosmetic products manufacturer | Unit 7, Westergate Business Centre, Westergate Rd | Mon–Fri 09:00–17:00 |
| Baroque Bespoke Jewellery | Jewellery designer | 9-10 Union St | Mon–Sat 10:30–17:00 |
| Bartholomews Tutorial College | College | Bartholomews Tutorial College, Prince Albert St | Mon–Fri 09:00–17:00 |
| Be The Fox | Advertising agency | 24B York Villas | Mon–Fri 09:00–17:30 |
| Beauty and Nail Bar | Nail salon | 11 Station Rd | Mon–Sat 10:00–17:00 |
| Belle Vue Cakery | Cake shop | Belle Vue Cottages | Daily 09:00–19:00 |
| Berkeley Hair Clinic | Hair transplantation clinic | 27 Middle St | Mon–Sat 09:00–20:00 |
| BESO Carpentry | Carpenter | H U S K Projects, Islingword Rd | Mon 08:00–20:00 |
| Bespoke Diamonds | Diamond dealer | Projects Beach, 9 Ship St | Mon–Sat 10:30–17:30 |
| Betapest - Brighton Pest Control | Pest control service | Canfield Rd | Mon–Fri 08:00–17:00 |
| Bikes of Brighton MOT Centre | Motorbike repair shop | Bell Tower Industrial Estate, Unit 1 Roedean Rd, Bell Tower Ind Est, 4 Roedean Rd | Mon–Sat 09:00–17:30 |
| Bismillah Spice of Life | Bangladeshi restaurant | The Level, 59-61 Ditchling Road ( By | Mon 17:30–22:00 |
| Black Sheep Utilities Ltd | Energy supplier | 125-135 Preston Rd | Mon–Thu 08:45–17:00 |