| Preston Park | Park and garden | Preston Rd | Daily Open 24 hours |
| Preston Park Tennis Courts | Tennis court | Preston Rd | Daily 07:00–20:00 |
| Pro Tint Studio - Residential and Commercial Window Film - Brighton & Hove | Window tinting service | 1 Wilmington Parade | Mon–Sat 08:00–18:00 |
| Promo Solved Ltd | Promotional products supplier | 4 Village Cl | Thu 08:00–18:30 |
| Purebred French Bulldogs & Border Collie | Dog breeder | Richmond Parade | Daily Open 24 hours |
| QPP Quick Print Pro | Copy shop | 36 Cheltenham Pl | Mon–Fri 10:00–17:00 |
| Racing Experts UK | Betting agency | 122 Carden Ave | Daily Open 24 hours |
| Raj Rayan - Partner of James & Co solicitors LLP. Immigration, Nationality & Refugee Law specialist | Immigration lawyer | 3rd Floor, Queensberry House, 106 Queens Rd | Mon–Fri 09:30–17:00 |
| Ranch, American Diner | American restaurant | 76 Western Rd | Thu 09:00–23:00 |
| Randstad Engineering Recruitment Agency - Brighton | Job centre | 1st Floor, Castle House, Sea View Way | Mon–Fri 07:30–17:30 |
| Rarekind Records | Record shop | 104 Trafalgar St | Mon–Sat 11:00–18:00 |
| RB Trophies & Engraving | Engraver | 372 Carden Ave | Mon–Fri 09:00–17:30 |
| Recovery Project | Addiction rehabilitation centre | 10 Ditchling Rise | Mon–Fri 08:30–17:00 |
| Red Tower Tattoos & Piercings | Body piercing shop | 114 Church St | Tue–Thu 10:30–17:00 |
| Redroaster | Brunch restaurant | 29a New Rd | Thu 09:00–22:00 |
| Reiki & Energy Healing in Brighton - Sunrise Healing | Reiki therapist | 20-22 Gloucester Pl | Daily Open 24 hours |
| relovestuff | Furniture repair shop | 27 Park Crescent Terr | Mon–Sat 09:00–17:00 |
| RePrint Brighton | Commercial printer | 47 Highcroft Villas | Mon–Fri 08:30–17:00 |
| Responsible Travel | Travel agent | Nile St | Mon–Thu 09:00–18:00 |
| RideTo | Motorcycle driving school | 15 - 17 Middle St | Daily 08:30–17:00 |
| Risk to Live Sex Therapy | Sexologist | Ground Floor, 41 Little Preston St | Mon–Fri 11:00–20:00 |
| Rittman Mead Consulting Ltd. | Computer consultant | Fl 6, Tower Point, 44 North Rd | Mon–Fri 09:00–17:00 |
| RJ Meaker Fencing Ltd | Fence supply shop | 11 Middle St | Mon 07:30–17:00 |
| Rocket Projects Office Refurbishment | Shopfitter | 47 Middle St | Mon–Fri 09:00–17:30 |
| Roguecycle | Indoor cycling | Cycle Hub at, Station | Sat 08:00–13:00 |
| Roots Research Ltd | Market researcher | 50 Grand Parade | Mon–Fri 09:00–17:30 |
| Roundabout Nursery | Family service center | 178 Whitehawk Rd | Mon–Fri 08:00–18:00 |
| Royal Alexandra Children's Hospital | Children's hospital | North Dr | Daily Open 24 hours |
| Run Company | Running shop | 16-17 Queens Rd | Mon–Sat 09:30–18:00 |
| Rustico Neapolitan (Brighton) | Neapolitan restaurant | 300 Ditchling Rd | Mon–Tue 16:00–21:00 |