| Dishoom Permit Room Brighton | Indian restaurant | 32 East St | Thu 09:00–22:00 |
| Ditto Fabrics Ltd | Fabric shop | 21 Kensington Gardens | Mon–Sat 10:00–17:30 |
| DMH Stallard | Property lawyer | The Portland Building, 27-28 Church St | Mon–Fri 09:00–17:00 |
| Dowse | Homewares shop | 37 Gardner St | Daily 11:00–17:00 |
| Dragonhead Fencing Club | Fencing school | Old Boat Community Centre, 172 Carden Hill | Tue 15:30–20:00 |
| DripDry Gas Heating & Plumbing | Gas company | 17 Woodland Way | Mon–Fri 08:00–17:00 |
| Driveway Cleaning Sussex | Pressure washing service | 27 St Luke's Rd | Daily Open 24 hours |
| Droneworks 360 | Drone service | No. 17, The Edge, Pankhurst Ave | Mon–Fri 08:00–18:00 |
| drumbox Drum Tuition | Drum school | 1 Roedean Rd | Mon 10:00–20:00 |
| Dry Red Press Greeting Cards | Greetings card shop | 100 Church St | Thu 09:30–16:30 |
| East Brighton Amateur Boxing Club | Boxing club | The Crew Club, 26 Coolham Dr | Tue 18:00–21:00 |
| East Street Tap | Pub | 74 East St | Mon–Wed 12:00–02:00 |
| Easy Clean Sussex Ltd | Cleaners | 23 Coombe Terrace | Daily 09:00–17:00 |
| Economy of Brighton | Craft shop | Unit 1, Westergate Business Park, Westergate Rd | Thu 09:00–17:00 |
| Elena Gelibter | Nutritionist | 10 Baden Rd | Mon–Fri 09:00–05:00 |
| Elite Asbestos Removal Brighton Ltd | Asbestos testing service | 302A Ditchling Rd | Mon–Sat 08:00–19:00 |
| Elite Law Solicitors Brighton | Legal services | Queensberry House, 106 Queens Rd | Thu 09:00–17:00 |
| Embers | European restaurant | 42 Meeting House Ln | Thu 17:00–23:00 |
| Emmanuel International Groceries | Indian grocery shop | 1A Whitehawk Rd | Mon 07:30–13:00, 14:00–21:30 |
| Endura Flags Ltd | Flag shop | 1 Northfield Way | Mon–Fri 10:00–16:30 |
| Engage Health Group | Employee Benefits & Health Insurance | Health insurance agency | 1st Floor, 26 Stroudley Rd | Mon–Fri 09:00–17:00 |
| Equity Inspiring Learning Ltd | Tours | 3rd Floor, 100-101 Queens Rd | Mon–Fri 09:00–17:15 |
| Era Brighton | Antique furniture shop | 92 Trafalgar St | Mon 10:30–16:30 |
| Erasmus+ Brighton Ltd | Foreign exchange students organization | 15-17 Middle St | Daily Open 24 hours |
| Escape the Vault - Escape Room | Escape room centre | London Rd | Mon–Sat 10:00–00:00 |
| European Shiatsu and Acupuncture School (ESAS) - Brighton | Acupuncture school | 28-29 Bond St | Mon–Thu 09:00–17:00 |
| Eventive Games | Event technology service | 21 Harrington Rd | Mon–Fri 09:00–17:30 |
| Evermade | Poster shop | Ste 1, 2nd Floor, Vantage Point, New England Rd | Daily 10:00–18:00 |
| EWS-Sussex | Waste collection service | 118 Brentwood Rd | Mon–Thu 07:30–17:30 |
| Executive Car & Limousine Hire Sussex | Limousine service | 21 Abinger Rd | Daily 08:00–21:00 |