| TopG Wings Brighton | Fast food restaurant | 101 Western Rd | Thu 12:00–23:00 |
| Triad Classic Motorbikes | Motorbike dealer | 64 Old London Rd | Mon–Fri 10:00–13:00, 14:00–17:00 |
| Twisted Lime | Cocktail bar | 1 Middle St | Mon 17:00–01:00 |
| UK Catering Ltd | Catering supplier | 304, Vantage Point, New England Rd | Thu 09:00–17:00 |
| Unique by Design Children's Boutique | Boutique | 6 St Cuthmans Cl | Wed 09:00–21:00 |
| Upledger Institute UK | Educational institution | 196 Surrenden Rd | Mon–Fri 09:30–15:30 |
| Utility | Homewares shop | 28A North Rd | Mon–Fri 10:30–17:30 |
| Vanlife Services | Caravan repair shop | Regency Square | Mon–Fri 08:00–17:00 |
| Vincent Dance Theatre | Theatre company | Moulsecoomb Hall, Lewes Rd | Mon–Thu 09:00–17:00 |
| Weddel Swift Distribution Ltd | Meat wholesaler | Units 3-4, Meat Market, Upper Hollingdean Rd | Mon 04:00–12:00 |
| Wedding Rings Direct Head Office | Jewelry manufacturer | Unit 1, Level 4, New England House, New England St | Mon–Fri 09:00–17:00 |
| Wellesbourne | Residents association | Preston Rd | Mon 09:00–17:00 |
| Westdene Butchers | Butchers | 34 Eldred Ave | Mon 09:00–16:00 |
| Wimpole Hair Transplant Clinic Brighton | Hair transplantation clinic | Queensberry House, 106 Queens Rd | Mon–Fri 08:00–20:00 |
| With Woman Clinic | Women's health clinic | 124 Carden Ave | Mon 10:00–14:30 |
| Wolfox Coffee Express | Coffee stand | Queens Road Inside the Train Station | Mon–Fri 06:00–19:00 |
| Woodingdean Well | Historical landmark | 509 Warren Rd | Daily Open 24 hours |
| Yates Scaffolding Services | Scaffolding service | 161-163 Preston Rd | Tue Open 24 hours |
| YEAH laser | Laser cutting service | Unit 13, Level 4, Vantage Point, New England Rd | Mon–Fri 12:00–17:00 |
| York & Elder | Apartment building | 52 New England St | Thu Open 24 hours |
| YouthTheGap | Educational consultant | 40 Halland Rd | Daily Open 24 hours |
| YOYO Yard | Sauna | 36 Cheltenham Pl | Mon 07:00–21:00 |
| Zurich Insurance | Insurance agency | 2nd Floor, Lanchester House, 67 Trafalgar St | Thu 09:00–17:00 |
| 001 Translations - Brighton Certified Translation Services | Translation service | 91 Western Rd | Daily 08:00–22:00 |
| 67 Centre Young Futures Hub | Youth centre | Moulsecoomb Way | Mon–Fri 09:00–21:00 |
| Agile IP - Patent and Trade Mark | Patent lawyer | Blanche House, Dyke Rd | — |
| Albion Community Garden | Community garden | Albion St | Daily 11:00–17:00 |
| Anchor Leather | Leather goods manufacturer | 17 Bennett Rd | Mon–Fri 08:30–18:30 |
| Babyprints Brighton | Baby shop | 20 Mill Ln | Mon–Fri 09:00–15:00 |
| Baka Neko Brighton | Anime club | 20 Marshalls Row | Fri–Sat 10:00–17:00 |