| Ronin-Pentest - Cyber Security Consultancy | IT security service | 103A Albion Hill | Mon–Thu 09:00–17:00 |
| Royal Sussex County Hospital | General hospital | Eastern Rd | Daily Open 24 hours |
| Run Rooster peri peri Brighton | Chicken shop | 37 Ditchling Rd | Thu 16:00–23:00 |
| Runway East Brighton | Office Space | Office rental agency | 50 New England St | Thu 09:00–17:00 |
| Ruth Hughes Speech Therapy | Speech pathologist | 26 Withdean Cres | Mon–Fri 09:00–17:00 |
| Saint Thomas More's Catholic Church | Catholic church | Braybon Ave | Wed 10:00–11:00 |
| Salt Shed at The West Pier | Barbecue restaurant | 91-96, Kings Road Arches | Thu 17:00–21:00 |
| Sapphire Eye Care Brighton | Ophthalmology clinic | Warren Rd | Wed–Thu 06:30–20:30 |
| SAVVY Female Fitness | Women's personal trainer | 192 Old Shoreham Rd | Mon–Fri 05:30–19:30 |
| Savvy sleep coach | Sleep clinic | 14 Port Hall Pl | Daily 09:00–21:00 |
| Scaffolding Sussex | Scaffolding service | Johnson Bank, 37 Johnson's Bank, Wellington Rd | Sun Open 24 hours |
| School of Business and Law, University of Brighton | Business school | Elm House, University of, Lewes Rd | Fri 08:00–20:00 |
| School of Tai Chi Chuan and Internal Arts | Tai chi school | West Hill Hall, Compton Ave | Fri 10:00–12:00 |
| Sherlock Studio | Branding agency | Unit 4, Diplocks Yard, 73 North Rd | Thu 08:30–17:30 |
| Shopmobility | Mobility equipment supplier | Red car park, Level P3 Churchill Square Car Park 1 (Orange, Grenville St | Mon 09:00–17:00 |
| Silicon Brighton | Business networking company | PLATF9RM, Floor 5 & 6, Tower Point, 44 North Rd | Thu 09:00–17:30 |
| Simplify and Style | Professional organiser | 48 Prestonville Rd | Mon–Fri 09:00–17:00 |
| Skinned Alive Tattoo | Tattoo shop | 24 Church St | Fri 11:00–17:00 |
| Smarthoard Ltd | Structural engineer | 83 Queens Rd | Thu 08:30–17:30 |
| Smarty Lamps | Lighting manufacturer | Online Shop Smartdecostyle.com, 25 Reading Rd | Mon–Sat 10:00–18:00 |
| Smokers Heaven | Tobacco shop | 10 Queens Rd | Mon–Sat 09:00–20:00 |
| Snagging inspections Brighton | Building inspector | 7-9 Windlesham Ave | Mon–Fri 08:30–19:00 |
| Solo Nutrition Ltd | Vitamin & supplements shop | Unit 9 Freshfield Ind Estate, Stevenson Rd | Thu 09:00–17:00 |
| Southern Water | Waste transfer station | Lewes Road Falmer | Mon–Fri 07:00–21:00 |
| Sparrows Nursery Ltd | Primary school | Benfield Primary School, 255 Old Shoreham Rd | Mon 08:45–15:15 |
| Specialist Herbal Supplies | Herbal medicine shop | Portslade Hall, 18d Station Rd | Mon 09:00–17:30 |
| Sprabuild | Precision engineer | Argyle Stables, 21b Campbell Rd | Mon–Thu 08:00–17:00 |
| Sprinkles Gelato Brighton | Dessert restaurant | 33 West St | Thu 12:00–00:00 |
| Sushimania Brighton | Sushi restaurant | 15-17 Middle St | Tue 12:00–23:00 |
| Sussex Cancer Fund | Charity | Royal Sussex County Hospital | Mon–Fri 08:00–15:00 |