| The Telemarketing Company | Telemarketing service | 26 & 27 Regency Square | Mon–Fri 08:30–17:00 |
| The Theatre Workshop Stage School | Drama school | High School, Montpelier Rd | Tue–Thu 09:30–17:30 |
| The Wellbeing Practice | Child psychologist | Sundial House, 111 Queens Rd | Mon–Fri 09:30–17:30 |
| The Womens Hormone Clinic | Women's health clinic | 93 Windmill Dr | Mon–Fri 09:00–17:30 |
| The Wood Store Brighton (Brighton & Hove Wood Recycling Project) | Furniture maker | Ground Floor, Oakley House, Edward St | Thu 09:00–17:30 |
| TheSkinVet & ThePhotoVet | Dermatologist | 17 Varndean Holt | Mon–Fri 09:30–14:30 |
| TheVegPieCollective | Food producer | 60 Florence Rd | Mon–Sat 09:00–17:30 |
| Thom Mills Drums | Recording studio | 25 Albion St | Daily 09:00–18:00 |
| Thomas Locksmiths | Key duplication service | 97 Portland Rd | Daily Open 24 hours |
| Three EEE's TCG Shop | Trading card shop | Unit 30, Downstairs, Marshalls Row | Mon–Wed 11:00–18:00 |
| ThreeDeeDave - 3D Print and 3D Scanning Service | Digital printing service | Vantage Point | Mon–Thu 09:00–17:30 |
| Tidy Print | Screen printing shop | Level 4, South, New England House, Unit 13 New England St | Mon–Sat 10:30–18:00 |
| Time2shine | House cleaning service | 68 Bates Rd | Mon–Fri 07:30–19:00 |
| To Be Worn Again Vintage Shop | Consignment shop | 12 Kensington Gardens | Thu 11:00–17:00 |
| Toby Tiger | Baby clothing shop | 11 Gardner St | Mon–Sat 10:00–18:00 |
| Tom Brown (StoneMind Studio Ltd) | Stone carving | 6 St George's Mews, Brunswick Sq | Daily 09:00–17:00 |
| Tom Fell Studio | Blacksmith | Mile Oak Farm, Mile Oak Rd | Mon–Fri 09:00–17:00 |
| Tori Bond Celebrant - Sussex Wedding and Funeral Celebrant | Funeral celebrant service | Franklin Rd | Daily Open 24 hours |
| Total Building Control Brighton | Building inspector | Unit 1w, Waterside House, Basin Rd N | Mon–Thu 08:30–17:00 |
| Trojan Asphalt Flat Roofing Specialists | Asphalt contractor | 39 Wilmington Way | Mon–Sat 07:00–19:00 |
| Tungsten Training Centre | Training centre | Freeman House, Ellen St | Mon–Fri 08:00–16:00 |
| Two Feathers | Native American goods shop | 11 Kensington Gardens | Thu 10:00–18:00 |
| UK Transcription | Typing service | Media Centre, Middle St | Mon–Fri 09:00–17:00 |
| UnBarred Brewery & Taproom | Brewery | 19-23 Elder Pl | Mon 16:00–23:00 |
| Unity Fertility | Fertility clinic | Therapy Rooms, Kemp town House, 72 Carlton Hill | Tue–Sat 08:00–19:00 |
| University of Brighton Careers and Employability Team | Student career counseling office | University of, Cockcroft Building, Careers Office, First, Lewes Rd | Mon–Thu 09:30–17:00 |
| Uplink Electronics | Electronics retail and repair shop | 70 London Rd | Sat 10:00–18:00 |
| Urban Outfitters | Clothing shop | Unit MS6, Cranbourne St | Thu 10:00–20:00 |
| Valley Therapy | Mental health services | Toronto Terrace | Mon–Thu 08:00–18:00 |
| Vapex Vending | Vending machine supplier | 1 New Rd | Mon–Sat Open 24 hours |