| Efes Restaurant | Turkish restaurant | 278-280 Glossop Rd | Daily 11:00–22:00 |
| Ego Sheffield City Centre | Restaurant | 88 Surrey St | Daily 11:00–23:00 |
| Elements Laundry & Dry Cleaners | Laundry service | 6 Wostenholm Rd | Mon–Sat 10:00–17:00 |
| Emergency Trauma Cleanup Ltd - ETC247 | Cleaners | 36 Parklands Ave | Daily Open 24 hours |
| End Time Evangelism Ministries | Non-profit organisation | 1 Tyler St | Wed 18:45–21:30 |
| Eric Eyre Funeral Service | Funeral home | Mortomley House, 2 & 4 Mortomley Ln | Daily Open 24 hours |
| Espiritu Cacao | Chocolate artisan | 18 Millmount Rd | Mon–Sat Open 24 hours |
| Expert Roofers Syk Limited | Roofing contractor | 12 Wharton Ave | Daily Open 24 hours |
| F Butcher & Son Ltd | Funeral home | 34 Lordens Hill | Daily Open 24 hours |
| F&F Clothing | Baby shop | 1 Savile St, Spital Hill | Mon–Sat 08:00–22:00 |
| Fairbank Investments Limited | Property investment | Fairfield Business Park, Green Road | Daily Open 24 hours |
| Fancy Fries Mobile Caterer | Mobile caterer | Chancet Wood Cl | Daily Open 24 hours |
| FastRank GBP | Local SEO, Search Ads & Digital Marketing | Advertising agency | 1 Surrey St | Daily Open 24 hours |
| Finningley Lodge Sheltered Accommodation - Sanctuary Housing Association | Sheltered housing | Victoria Cl | Daily Open 24 hours |
| FONE TECH - Sheffield | Mobile phone repair shop | Kiosk 2 The Moor | Mon–Sat 09:00–19:00 |
| For Fish Sake Ltd | Fishmonger | 49 Middlewood Rd | Tue–Sat 09:00–17:00 |
| Forest Lodge | Psychiatric hospital | 5 Forest Cl | Mon–Fri 18:00–20:30 |
| Forest Reach boarding cattery | Pet boarding service | 172 Swinston Hill Rd | Mon–Sat 10:00–12:00, 16:00–18:00 |
| FotoGroupMedia | Photography studio | 9 Mordaunt Rd | Daily Open 24 hours |
| Foundry Coffee Roasters | Coffee roastery | Cutlery Works, 79-101 Neepsend Ln | Mon–Fri 09:00–16:00 |
| Friesday Belgian Fries | Belgian restaurant | 280 Ecclesall Rd | Mon–Thu 11:00–22:00 |
| Funhouse Comedy Club in Sheffield | Comedy club | New Barrack Tavern, 601 Penistone Rd | Daily Open 24 hours |
| Gift Hampers Sheffield | Gift basket shop | Winston Business Park, Churchill Way | Daily Open 24 hours |
| Glenmill Carpets & Beds Ltd | Carpet retail shop | 38 Spital Hill | Mon–Sat 09:00–17:30 |
| Goldsmiths North | Exhibition and trade centre | Cutlers Hall, 7-15 Church St | Fri–Sat 10:00–17:00 |
| Grand Kerala Restaurant | Indian restaurant | 33 Carver St | Daily 08:00–23:00 |
| Granite & Quartz Kitchen Worktops – Expert Supply & Fit | Sheffield, Nottingham, Lincoln & London | Granite supplier | 25 Cardwell Ave | Daily Open 24 hours |
| Gravel Pit Shop | Nursery | 329 Abbeydale Rd | Wed–Sat 11:00–18:00 |
| Graves Park | Park and garden | Hemsworth Rd | Daily Open 24 hours |
| Green Spirit Horticulture | Hydroponics equipment supplier | Unit 5, 230 Woodbourn Rd | Mon–Fri 10:00–18:00 |