| Aquathlete Ltd | Swimming instructor | Rear of Stoney Cross, 102a Nottingham Rd | Mon 09:30–19:30 |
| ARB & Grounds | Lawn mower shop | St Georges Court Breadsall Island, Alfreton Road | Fri 08:00–17:00 |
| Ashfen Windows and Doors Ltd | Window supplier | Unit 15 Dunstall Park Rd | Mon–Fri 07:00–15:30 |
| Assured Vehicle Movement & Storage | Delivery service | Private Road, Wincanton Cl | Thu 08:00–17:00 |
| Aston-on-Trent Post Office - Convenience Store | Post office | 1 Derby Rd | Mon–Tue 06:00–21:00 |
| Atkins Wallcare Damp Proofing Derby | Water damage restoration service | 11 Meadow Way | Mon–Fri 09:00–18:00 |
| Audion Speedbags | Packaging company | 4, Centurion Way, business park | Thu 09:00–17:30 |
| Audrey Drive Children's Centre | Childcare agency | 3 Audrey Dr | Tue–Fri 09:00–17:00 |
| Aura Asbestos Survey Derby Ltd. | Asbestos testing service | 187 Normanton Rd | Mon–Sat 08:00–19:30 |
| Aurora Fabrications Ltd | Metal fabricator | Unit 4, Draycott Mill Market Street, Draycott, Mills Cl | Mon–Fri 08:00–18:00 |
| B.R Removals Derby | Removals service | Ste 1, Acorn Place, Alfreton Rd | Thu 07:00–18:00 |
| Balanced Bodies | Osteopath | 14 Vernon St | Mon–Fri 09:00–20:00 |
| Bates Weston | Accountant | The Mills, 49 Canal St | Mon–Fri 09:00–17:00 |
| Beau Belles (derbyshire) Ltd | Bridal shop | 11 Derby Rd | Mon–Wed 10:00–18:00 |
| Bedazzled Boutique | Ladies' clothes shop | 30 The Strand | Mon–Tue 10:00–17:00 |
| Beer Metropolis Wholesale | Beer distributor | Unit 8B, Old Hall Mill Business Park Alfreton Rd | Mon–Thu 08:00–16:30 |
| Belcan International Ltd | Engineering consultant | Kelvin House, RTC Business Park, London Rd | Mon–Fri 08:30–17:00 |
| Benz Bavarian Bodyshop | Vehicle repair shop | Scropton Rd | Mon–Fri 08:00–17:00 |
| Berkeley Hair Clinic - Derby | Hair Transplant Clinic | Hair transplantation clinic | 79 Rykneld Rd | Mon–Sat 08:00–20:00 |
| Best Distributions | Distribution service | 29 Stanage Grn | Mon–Fri 09:00–18:00 |
| Bevvys2u | Off licence | 8 | Mon–Sat 06:00–22:00 |
| Beyond Clean | Window cleaning service | 166 Greenwich Dr S | Mon 08:30–16:00 |
| Big On Bikes | Bicycle repair shop | Duffield Rd, The Hub | Mon 09:30–17:00 |
| Binder Creations Ltd | Stationery manufacturer | 22 Sir Francis Ley Industrial Park, Shaftesbury St S | Mon–Fri 08:00–17:00 |
| Bionical Solutions | Internet marketing service | The Piazza, Mercia Marina, 8 Findern Ln | Fri 08:00–18:00 |
| Blue i Properties | Apartment letting agency | 30a Ashbourne Rd | Mon–Fri 09:00–17:00 |
| Bluebell Dairy Ice Cream Farm | Children's farm | Locko Rd | Mon 10:00–16:00 |
| Blueprint Document Solutions | Printing equipment supplier | Marble Hall, 80 Nightingale Rd | Mon–Fri 08:30–17:00 |
| Boditronics | Sports nutrition shop | 3a Harrison Court | Mon–Fri 08:00–17:00 |
| Bodycare | Health and beauty shop | Debenhams Westfield Derby Shopping Centre, 5 Traffic St | Mon–Wed 09:00–18:00 |