| Early Life Ultrasound Centre | Pregnancy care centre | 16, Ormond Terrace, Regent St | Mon 09:30–21:00 |
| East Glos Club: Tennis, squash, padel, racketball | Sports club | Old Bath Rd | Mon–Fri 09:00–19:45 |
| Eden Property Student Houses Cheltenham | Student accommodation centre | 49 Marle Hill Parade | Mon–Fri 09:00–17:00 |
| Edward George Tailoring | Bespoke tailor | The Old Prison, 1st Floor, A429 | Mon–Fri 09:00–19:00 |
| EH Activewear | Sportswear shop | 33 Tennyson Rd | Mon 08:00–20:00 |
| Elaine Morane Makeup | Make-up artist | 1 Chiltern Rd | Mon 09:30–17:00 |
| Elite Towbars and Windscreens Ltd | Auto electrical service | 10 Nettleton Rd | Mon–Fri 09:00–17:00 |
| Elkstone Studios | Co-working space | Elkstone, Studios | Daily 09:00–17:00 |
| Elliott Aggregates Ltd | Aggregate supplier | Irish Butts/Stoke Orchard Rd | Mon–Fri 07:30–17:00 |
| Elwood Computer Services Ltd | Computer service | Star Court, Pittville Circus Rd | Mon–Thu 08:00–18:00 |
| Embrace Building Wraps | Advertising agency | Hope Cottage Barn, Chapel St | Mon–Fri 09:00–18:00 |
| Ermin | Equipment rental agency | Swindon Rd | Fri 07:30–17:00 |
| Ethical Addictions Coffee Roasters | Coffee wholesaler | Bamfurlong Industrial Park, Coffee Roastery, 21a | Mon 09:00–17:00 |
| Evenlode Films and Productions Ltd | Film production company | Evenlode Films and Productions, Parker Court, Knapp Ln | Mon–Fri 08:00–20:00 |
| Evergreen House | Mental health clinic | Charlton Ln | Mon–Fri 09:00–17:00 |
| Eyre Plastering & Building Services Ltd | Plasterer | 20 Three Sisters Ln | Mon–Thu 08:00–18:00 |
| Faber Building Limited | Flooring contractor | Wellington St | Mon–Sat 08:00–18:00 |
| Fireplace Gallery Fires & Stoves - Cheltenham | Fireplace shop | Unit L, Runnings Road Kingsditch Trading Estate | Mon–Fri 09:00–17:00 |
| For The Saints | Espresso bar | 22 Montpellier Walk | Mon–Fri 08:00–17:00 |
| Forgeability Ltd | Metal fabricator | Unit 8, Orchard Industrial Estate | Mon–Fri 08:00–17:00 |
| Fosseway Tool Hire Ltd | Gas company | Bourton Link, Unit 4 Willow Ct | Mon–Fri 07:30–17:00 |
| Four Anjels | Wholesale bakery | Business Park, Unit 10 Furlong Park | Daily 06:00–22:00 |
| Future Athletes | Nutritionist | 4 Lower Mill St | Mon–Fri 09:00–19:30 |
| Gallagher Retail Park | Shopping centre | Tewkesbury Rd | Mon–Sat 09:00–20:00 |
| Gas Junky Motorcycles | Motorbike repair shop | 16 Alstone Lane Trading Est, Alstone Ln | Mon–Fri 10:00–18:00 |
| Ginger & Garlic | Korean restaurant | 334 High St | Mon–Tue 12:00–21:30 |
| GJC Plumbing and Heating Ltd | Gas engineer | 48 Pilley Cres | Mon–Fri 08:00–17:00 |
| Gloster Aviation Medicals Ltd | Occupational health service | Unit 4, Bamfurlong Business Park | Mon–Wed 08:30–17:00 |
| Gloucestershire Clean Ears | Health consultant | Sapphire Rd | Tue 18:30–20:30 |
| Gloucestershire Knee Clinic - Cheltenham | Orthopaedic surgeon | Nuffield health cheltenham hospital, Hatherley Ln | Mon–Fri 08:00–20:00 |