| All is GOOD | Deli | Unit B, & C, 1 St Mary's St | Mon 10:00–16:00 |
| Amigo Kids | Language school | Penistone Leisure Centre | Mon–Fri 10:00–18:00 |
| Argo Feeds Ltd | Pet food and animal feeds | Kirkwood Corn Mills, Sheffield Rd | Mon–Fri 08:00–17:30 |
| Cafe Creme | Coffee store | 9 High St | Mon 08:30–16:30 |
| Cleaning Angels Yorkshire Ltd | House cleaning service | suite 3, Crown House, Market St | Mon–Fri 09:00–17:00 |
| Ear Again Limited | Local medical services | Rotherwood, The Green | Mon–Fri 07:00–16:30 |
| JH Milnes Ltd | Agricultural engineer | New Chapel Farm, Chapel Ln | Mon–Fri 08:00–17:30 |
| Melvyn Carr Ltd | Farm equipment supplier | 55 Huddersfield Rd | Mon–Fri 08:00–17:00 |
| My Work Gear | Work clothes shop | Fairfield Business Park, Green Rd | Mon 09:00–17:00 |
| Penistone Church Football Club | Football club | The Simply Log Cabins Memorial Ground, Church View Rd | Mon 18:00–23:00 |
| RFA-TECH LTD T/A 7 Steel Service | Construction material wholesaler | Bullhouse Works, Manchester Rd | Mon–Fri 08:30–16:30 |
| Springvale Household Waste Recycling Centre, Penistone | Waste-management service | Sheffield Rd | Daily 09:00–16:00 |
| Stonehouse Financial Services Ltd | Financial advisor | 13 Market St | Mon–Tue 09:00–16:30 |
| The Fire Place | Tienda de chimeneas | 3 Thurlstone Rd | Tue–Fri 10:00–16:00 |
| Valley Kitchen Catering | Caterer | Westfeild House Farm | Daily Open 24 hours |
| White Pine Counselling & Wellness | Counsellor | 66 High St | Tue–Thu 08:00–21:00 |
| mydentist, High Street, Penistone | Dentist | 11-15 High St | Mon–Fri 09:00–17:30 |
| Persimmon Homes Hartcliff Meadows | Housing development | New Chapel Rd | Daily 11:00–18:00 |
| GoPromotional Products - Sheffield | Promotional products supplier | 1, Regent Court, St Mary's St | Thu 08:00–18:00 |
| Q E D Financial Associates Ltd | Financial advisor | Regents Court, St Mary's St | Mon 09:00–17:00 |
| Swim Play Academy | Swimming school | Willow Lodge | Fri 09:00–20:00 |
| Frillies | Lingerie shop | 4 Bridge St | — |
| Grounded Groundworks | Excavating contractor | 31 Saunderson Rd | Mon Open 24 hours |
| Little Leopards Baby Massage & Yoga | Wellness programme | Unit G, St Mary's St | — |