| O'RIORDAN - Film Production & Camera Rental Company - Brighton and Hove. | Film production company | 2 Market Sq, Circus St | — |
| ONE PIECE CARD GAME Official Shop | Trading card shop | 26 North St | Thu Open 24 hours |
| Oriental Select | Asian grocery shop | 79-80 Western Rd | Sun 10:00–18:00 |
| Paul Reed Architects Ltd | Architecture firm | Studio Greenhouse, 18 Bond St | — |
| Pepper-Pot Nursery and Pre-school | Pre-school | 39 Whippingham Rd | — |
| PLATF9RM Brighton - Coworking, Offices & Events | Co-working space | Floor 5 & 6, Tower Point, 44 North Rd | — |
| Plug-In Media | Media company | 3rd Floor, St Augustine's Centre, Stanford Ave | — |
| Preloved of Brighton | Second-hand clothing shop | 267A Preston Drove | — |
| Premier Shredding Brighton | Shredding service | Tower Point, 44 North Rd | Mon 08:30–17:00 |
| Pumpkin Patch Nursery | Nursery school | 37 Preston Drove | — |
| Quartz Payroll | Payroll service | 7th Floor, Telecom House, 125-135 Preston Rd | — |
| Richard Cleaning products supplier | Cleaning products supplier | 40 Grand Parade | Thu Open 24 hours |
| Root Ayurveda | Wellness centre | 1, The Offices, 10 Fleet St | — |
| Sage's Sporting Store | Fitness equipment wholesaler | London Rd | Daily Open 24 hours |
| Sector (UK) | Fence contractor | 130 Vale Ave | — |
| Sock Goblin Yarn | Handicraft | Open Market | Fri–Sat 09:00–17:00 |
| Solar Rise | Solar energy equipment supplier | 14 Barnett Rd | — |
| Source Military The Bunker | Army & navy surplus shop | 74 Trafalgar St | Mon–Fri 10:30–17:00 |
| Stay True Gems | Cosmetic dentist | 2 North Rd | Sat 11:00–17:00 |
| The Bastion Grapple Co. | Jiu jitsu school | International House, Queens Rd | Sat–Sun 07:00–20:00 |
| The Bathroom Showroom | Bathroom supply shop | Unit 4, City Plumbing Supplies, A to C, Freshfield Way | Mon 07:00–17:00 |
| The Eyrie Football Pitch | Football pitch | Haybourne Rd | Daily Open 24 hours |
| The Famous King of Rock | Sweet shop | 40 Kings Rd | Daily 09:30–19:00 |
| THE FLINT WALL Cọ | General contractor | 159 Hartington Rd | — |
| The Forge Comedy Club | Comedy club | The Forge Comedy Club, 30 Cheapside | — |
| The Ledward Centre | Community centre | 14a Jubilee St | — |
| The Radiator Centre | Radiator shop | 69 Trafalgar St | Mon 09:00–17:00 |
| The Wig Workshop | Wig shop | Unit 23 Level 6, New England House, New England St | — |
| Time For Kimchi | Korean food shop | 19 Church St | — |
| TMF VAT Services Limited | Business administration service | 3rd Floor, Queensberry House, 106 Queens Rd | — |