| Detag Graffiti Removal and Prevention | Graffiti removal service | 17 Coleman St | Mon Open 24 hours |
| Dinnages Publishing | Publisher | No personal callers, 348 Carden Ave | Daily Open 24 hours |
| Dormitory | Linen shop | 101 Gloucester Rd | Thu 10:00–17:30 |
| Downs Baptist Church | Baptist church | Brighton and Hove, Brighton BN2 6BB | Mon 09:00–13:00 |
| Dukes Lane | Shopping centre | Dukes Ln | Mon 11:00–17:00 |
| E D R Heating Services Ltd | Heating equipment supplier | Flat 1, 26 Reigate Rd | Thu 08:00–18:00 |
| Easthill Glass House | Nursery | Easthill Park | Fri Open 24 hours |
| Ed Casswell Ophthalmology | Surgeon | Warren Rd | Fri 09:00–17:00 |
| Edgar & Wood Mechanical Services Ltd | HVAC contractor | Hove Enterprise Centre, Unit 12 Waterside House, Basin Rd N | Mon–Sat Open 24 hours |
| Eightfold Property | Property consultant | 86 Gloucester Rd | Thu 09:00–17:00 |
| Elite Music Management | Music management and promotion | Brighton and Hove, Brighton BN2 4WA | Mon–Fri 09:30–16:00 |
| Embodied Psychology | Psychologist | 18A Clermont Rd | Mon–Fri 09:00–17:00 |
| Emma Edelston | Stained glass studio | Eastend, Lower Ground Floor | Thu–Fri 09:00–17:00 |
| Energy Kidz After School Club - Brighton & Hove (Peter Gladwin Primary School) | Children's club | Peter Gladwin Primary School, Drove Rd | Mon–Fri 15:00–18:00 |
| Entacom | Security system installation service | 85 Boundary Rd | Mon 08:30–16:30 |
| ENV Surveys Ltd | Asbestos testing service | 12 Upper Hollingdean Rd | Fri 09:00–17:00 |
| Equilibrium Therapy Clinic Brighton | Pain control clinic | The Foundry, St George's Mews | Tue–Wed 10:00–17:00 |
| Espalier Ventures | Private equity firm | 1 Gloucester Pl | Mon–Fri 09:00–17:00 |
| Excel Training Brighton | School of computing | 8 Mulberry Cl | Daily 10:00–19:00 |
| Expression Insurance | Insurance company | 8-9 Ship St | Thu 09:00–17:00 |
| Eyesite Opticians & Hearing Care | Optician | 47/48 North St | Mon–Sat 09:00–17:30 |
| Factory Films | Video production service | 83 Queens Rd | Thu 09:00–18:00 |
| Felicity Fairy - Children's Entertainers | Party planner | 59B Ship St | Mon 09:00–22:00 |
| Finance Planning Group - Claire Lipscomb | Mortgage broker | 17 Mill Ln | Thu 08:00–20:00 |
| Food for Friends | Vegetarian restaurant | 17-18 Prince Albert St | Mon–Fri 09:00–22:00 |
| Formula Fragrances | Perfume store | 5 Kensington Gardens | Thu 10:00–18:00 |
| Framed By Leylani | Eyebrow bar | 69 Rose Hill Terrace | Mon 12:30–20:00 |
| Fresh Air Facilities | Air conditioning contractor | 10 Boyce's St | Thu 09:00–17:00 |
| From the Seed | Aromatherapy service | 85 Mackie Ave | Mon 11:00–18:00 |
| Fuel Kitchen | Eclectic restaurant | 2, The Parade, Valley Dr | Mon 12:00–23:00 |