| Lancing Marine | Marine supply shop | 51 Victoria Rd | Mon–Fri 08:30–17:30 |
| LaserZone | Tourist attraction | 76 West St | Mon–Thu 11:00–21:30 |
| Leigh Andersen Carpentry and Kitchens - Brighton | Kitchen renovator | 299 Cowley Dr | Mon–Fri 08:00–20:00 |
| LeoFrames | Picture framing shop | 70 North Rd | Tue–Sat 10:00–17:00 |
| Leonidas Chocolates Brighton | Chocolate shop | LEONIDAS, 52 Queens Rd | Thu 10:00–18:00 |
| Lewes Road Launderette & Drycleaners | Launderette | 82 Lewes Rd | Daily 09:00–19:00 |
| LFX Computer Repair | Computer repair service | Business Connection, 145 Islingword Rd | Thu 09:00–18:00 |
| Lindsey Elliott Coaching | Life coach | 39 Brangwyn Dr | Mon–Wed 09:00–17:00 |
| Little Darlings Closet | Children's clothes shop | 23 Brighton Square | Tue–Wed 10:00–17:00 |
| Little Jasmine Therapies and Spa | Massage therapist | 14 New Rd | Thu 10:00–19:00 |
| Lock Stock & Barrel | Locksmith | 63 Ditchling Rd | Sun Open 24 hours |
| London Name Plate Manufacturing Co | Manufacturer | Zylo Works, Marine View | Mon–Thu 09:00–17:00 |
| London Sperm Bank Brighton | Sperm bank | 169 Preston Rd | Mon–Fri 08:00–17:30 |
| Long Story Short | Design agency | 11 Jew St | Mon–Fri 09:00–17:30 |
| Longboards UK Brighton | Skateboard shop | 24 Kensington Gardens | Mon 10:00–18:00 |
| Love Luxury Events | Event planning service | Units 15 & 16, 3rd Floor, Vantage Point, New England Rd | Mon–Fri 09:00–17:00 |
| Love Your Workspace | Office furniture shop | Unit 4, Quayside Units, Basin Rd S | Mon–Fri 09:00–17:00 |
| Loveabell Ltd. | Tent rental service | 240 Queens Park Rd | Thu 09:00–17:00 |
| Loves2tan | Tanning salon | 4 Warren Way | Mon 09:30–18:30 |
| Lucy & Yak Brighton North Road | Clothing store | 79-80 North Rd | Mon–Sat 10:00–18:00 |
| LUST! Brighton & Hove's sex shop | Adult entertainment store | 43 Gardner St | Mon–Fri 11:00–18:00 |
| Luxefloor & co ltd | Flooring contractor | 5 Uplands Rd | Thu 07:00–19:00 |
| Luxury Watch Group | Watch shop | 3 North Rd | Mon–Fri 10:00–17:00 |
| M Jupp Construction | Home builder | 61 Graham Ave | Mon–Sat 07:00–20:00 |
| M T Asphalt Roofing Services Brighton | Roofing service | 35 Hawkhurst Rd | Mon–Fri 07:00–19:00 |
| M&C Stevens ltd | Jewellery buyer | New England House, New England St | Mon–Fri 09:00–16:30 |
| Maestoso Music/Spanish Guitars (visit by appointment only) | Distribution service | 15 Buxton Rd | Mon 09:00–20:00 |
| Mainlysilver | Wholesale jeweller | Suite 17, Citibase, 95 Ditchling Rd | Mon–Fri 09:00–17:00 |
| Man & Van in Brighton and Hove - Duchon Logistics | Removals service | 35 Carlyle Ave | Thu Open 24 hours |
| Mandalaman | Art | 43 Belmont St | Mon 12:00–16:00 |