| Discover Difference Psychology Clinic | Mental health clinic | The Square, St George's Square | Mon–Sat 09:00–17:00 |
| Djkit® | Tienda de material para DJ | Windle Works, Southampton Rd | Mon–Fri 10:00–17:00 |
| Domom | Interior fitting contractor | Nutsey Lane | Mon–Fri 09:00–16:30 |
| Dress To Go | Formal wear shop | Botley Mills | Tue–Thu 10:00–18:00 |
| Droplet Scientific Ltd | Medical technology manufacturer | Epsilon House, Science Park, Enterprise Rd | Mon–Fri 09:00–17:00 |
| Dukes Recovery ltd | Towing service | 38 Ashdene Rd | Daily Open 24 hours |
| Ear Say Wax Solutions | Otolaryngologist | 75 Northmore Rd | Wed 08:00–18:00 |
| Earl of Bedlam | Bespoke tailor | Stowe | Daily 09:00–18:00 |
| Eastern Nights | Indian restaurant | 56 Thornhill Park Rd | Sun 17:30–23:30 |
| Ecco Wash | Car wash | Antelope Park, Bursledon Rd | Daily 07:00–19:00 |
| Ecological Survey & Assessment Ltd | Environmental engineer | Ten Hogs House, Manor Farm Offices, Flexford Rd | Mon–Fri 08:30–17:30 |
| Elite Cleaning Services | Gutter cleaning service | 6 Whitwell | Thu 08:00–18:00 |
| Elite Yacht Covers Ltd | Boat cover supplier | Unit 3, Firefly Road, Hamble Point Marina | Mon–Thu 07:30–16:30 |
| Enfields Estate Agents Shirley Property Sales | Estate agent | 347 Shirley Rd | Mon–Thu 09:00–17:30 |
| Enhanced Care Services & Urgent Treatment Centre | Urgent care centre | Unit H9, Adanac Park, Adanac Dr | Mon–Tue 08:30–20:00 |
| Eric Robinson Solicitors - Hedge End | Lawyer | Vanbrugh House, Grange Dr | Mon–Fri 09:00–17:30 |
| Esprit Electronics Ltd | Electronics manufacturer | Mitchell Point, Ensign Way | Mon–Thu 08:30–17:00 |
| Event Themes Ltd | Event planning service | The Mitre Building, Botley Rd | Tue 09:00–16:00 |
| Evolifts Ltd | Lift service | International House, International Business Park, George Curl Way | Mon–Thu 08:30–17:00 |
| Excelitas UK Ltd. | Laser equipment supplier | Unit 26-32, Mitchell Point, 32 Ensign Way | Wed 09:00–17:00 |
| Exclusive Commercials Ltd | Van accessories shop | Langhorn Garage, Langhorn Rd | Mon–Sat 10:00–17:00 |
| Expert Implants | Dental implants provider | Beaufort House, Hamble Ln | Mon–Fri 08:30–17:00 |
| Express Weldcare Services (EWS) | Tienda de suministros para soldadura | Unit B3, Empress Park, Empress Rd | Mon–Thu 07:30–16:30 |
| EyeStyle Opticians and Hearing Centre- Locks Heath | Contact lenses supplier | Locks Heath Centre, 16 Centre Way | Mon 09:00–17:15 |
| F. H. Brundle Southampton | Steel supplier | 502 Millbrook Rd/Third Av | Mon–Fri 07:00–17:00 |
| Fadoua Aesthetics | Beautician | 33 Old Common | Wed–Thu 09:00–18:00 |
| FANCI Fine Jewellery | Jewellery store | Unit 50, New Forest Enterprise Centre, Chapel Ln | Tue 09:30–17:00 |
| Fawkes & Reece- Southampton | Job centre | Notebeme House, 84 High St, Town Quay | Mon–Fri 08:00–18:00 |
| Fencing Centre Garden Structures Cadnam | Fence supply shop | Dobbies garden centre, Southampton Rd | Mon–Tue 09:00–17:00 |
| Field Palmer Estate Agents | Real estate agency | 391 Shirley Rd | Mon–Fri 09:00–18:00 |