| Aivaf Limited | Model maker | 1 Orgreave Cl | Mon–Fri 08:00–16:00 |
| AJAC Supplies | Pharmaceutical products wholesaler | The Balance, 2 Pinfold St | Tue 08:30–17:00 |
| AJS Building & Property Maintenance Ltd | Building restoration service | Unit 2, 870 Penistone Rd | Mon–Fri 08:00–17:00 |
| Ale & Radio Indie Beer Shop | Cigar shop | 12b Station Rd | Tue–Wed 12:00–18:00 |
| All is GOOD | Deli | Unit B, & C, 1 St Mary's St | Mon 10:00–16:00 |
| All Rounder Cricket & Hockey | Cricket shop | 2-5, Penistone Trading Estate, 391 Penistone Rd | Mon–Tue 09:00–18:00 |
| All Seasons | Distribution service | 4A Parkway Rise | Mon–Sat 03:00–12:00 |
| Allison Mobile Hair and makeup | Hairdresser | Lyndhurst Rd | Mon–Tue 09:30–19:00 |
| Ambi-Heat HVAC Ltd | HVAC contractor | 17 Churchill Rd | Daily Open 24 hours |
| Amigo Kids | Language school | Penistone Leisure Centre | Mon–Fri 10:00–18:00 |
| Amy Claire Ltd | Dressmaker | 145 Chaucer Rd | Tue–Fri 10:00–19:00 |
| Anabolic | Pharmaceutical products wholesaler | 1 Fargate | Daily Open 24 hours |
| Anchor - St Clements Court | Housing association | St Clements Court, 10 Mauncer Ln | Mon–Fri 08:00–18:00 |
| Ancient Wisdom - Giftware Wholesale | Wholesaler | Europa Drive Affinity, Park | Mon–Fri 08:00–16:00 |
| Andy Green Hearing Aids & Ear Wax Removal | Hearing aid shop | J C Bird Optician, 41-43 Surrey St | Mon 10:00–16:00 |
| Andy's Smart Car Body Repair | Car restoration service | 41 Cramfit Cres | Mon–Fri 09:00–17:00 |
| Annie's | Fabric shop | 554 Abbeydale Rd | Mon–Sat 10:00–16:00 |
| Anston Medical Centre | Surgical center | 15 Quarry Ln | Mon–Fri 08:00–18:30 |
| Ant Marketing | BPO company | The Chambers, 14 Church St | Mon 06:00–20:00 |
| Apollo Residence | Student and Professional Accommodation | Furnished apartment building | 2 Furnival Square | Mon 09:00–17:30 |
| Apollon Greek Grill | Gyro restaurant | 529 Ecclesall Rd | Daily 10:00–00:00 |
| Arbourthorne Tiddlywinks Centre | Day care center | 7 Eastern Dr | Mon–Fri 08:00–18:00 |
| Argo Feeds Ltd | Pet food and animal feeds | Kirkwood Corn Mills, Sheffield Rd | Mon–Fri 08:00–17:30 |
| Armed Forces Careers Office - Sheffield | Military recruiting office | Townhead House, 10-14 Townhead St | Mon–Fri 09:00–17:30 |
| Arnold Laver Head Office Bramall Lane | Wood supplier | Bramall Ln | Mon–Thu 08:00–16:30 |
| Art For Wellness CIC | Art centre | 12 Lockwood Gardens | Mon–Fri 10:00–16:00 |
| Arthrex | Medical technology manufacturer | Unit 1, Bessemer Park, Shepcote Ln | Mon–Fri 07:30–17:30 |
| Arup | Design engineer | 9th Floor, 3 St Paul's Pl, Norfolk St | Mon–Fri 09:00–17:30 |
| Asphalt Grid Systems & Advanced Composites Limited | Road construction company | Butterthwaite Ln | Mon 08:00–17:00 |
| Aspire Hair Wigs | Wig shop | The Lifestyle Centre, High St | Wed–Sat 09:00–15:30 |