| Divino | Pizza restaurant | 2 Saracen Wy | Mon–Wed 12:00–16:00 |
| DLG Auto Services Peterborough | Vehicle repair shop | 17 Bakewell Rd | Mon–Fri 08:30–17:30 |
| DLS Medical | Medical equipment manufacturer | Premier House, Southgate Way | Mon–Fri 09:00–17:00 |
| Dogsthorpe Infant School | School | Central Ave | Mon–Fri 08:30–16:00 |
| Don Churro (Peterborough) | Churreria | A1m Motorway | Fri 10:00–23:00 |
| DOR 4x4 Parts | Motorsports shop | Unit 19, Bungalow Farm, Werrington Bridge Rd | Wed 09:00–16:00 |
| Dor-Jan Windows & Doors Ltd | Window supplier | Unit 17, 19 Fenlake Business Centre | Mon–Fri 08:30–17:30 |
| Drip Dry Productions | Recording studio | 27 Norfolk St | Mon–Sat 09:00–17:00 |
| Drive With L Driving School | Driving school | 35 Blackmead | Mon–Sat 07:00–19:00 |
| Dynamic Consultancy Solutions Ltd | Consultant | Ind Est, Unit 4 Benwick Rd | Mon 09:00–17:00 |
| E-Bound AVX Ltd | Security system supplier | 3-4, Routeco Business Park, Bakewell Rd | Mon–Fri 08:00–18:00 |
| E-PULSE STUDIO Peterborough | Sports nutrition shop | 46 Huntly Grove | Mon–Fri 08:00–20:00 |
| East Restaurant | Restaurant | Upper Deck Charters, Town Bridge | Mon–Sat 12:00–14:30, 17:00–22:30 |
| Eastern Garage Doors | Garage door supplier | Stuart House East Wing, St John's St | Mon 07:00–17:00 |
| Eastfield Discount Autospares & Window Tinting | Window tinting service | 34-36 Eastfield Rd | Mon–Fri 09:00–17:30 |
| Easy Cheesy Peterborough | Pizza takeaway | 3 Orton Ave | Daily 11:30–23:45 |
| Eco Innovation Centre | Conference centre | Eco Innovation Centre Peterscourt, City Rd | Thu–Fri 09:00–17:00 |
| Eden Hut Saunas UK | Sauna shop | W Lake Ave | Mon–Fri 09:00–19:00 |
| Edge Steam Cleaning | Blast cleaning service | 64 Portchester Cl, Park Farm Wy. | Daily Open 24 hours |
| Empsy Limited | Psychologist | Peterborough United Football Club, Empsy© Limited c/o:, Cambridgeshire, London Rd | Daily Open 24 hours |
| EPD Insulation Group LTD | Insulation materials shop | Finmere Park | Mon 07:30–17:00 |
| Ethical Arboriculture | Tree service | 134 Wingfield | Mon–Fri 09:00–17:00 |
| Eurocool Ltd | Air conditioning contractor | The Rookery | Thu 08:30–17:00 |
| Excel Auto Care Ltd | Vehicle repair shop | Holme Rd | Mon–Fri 08:00–17:30 |
| Express Print Millfield | Printing shop | Office 1, 2 Parliament St | Daily Open 24 hours |
| Fancy Frox | Dress shop | 118 Culley Ct | Mon 09:00–20:00 |
| Fengate Service Centre Ltd | Car battery shop | Unit A, A1, First Drove | Mon–Fri 09:00–18:00 |
| Ferry Meadows in Nene Park | Tourist attraction | Ferry Meadows Country Park, Ham Ln | Daily 06:30–18:30 |
| Ferry Meadows Visitor Centre | Visitor centre | Ferry Meadows in Nene Park, Nene Park, Ham Ln | Mon–Tue 10:00–17:00 |
| Firmin & Co | Estate agent | 48b Church Dr | Mon–Fri 08:30–17:00 |