| ASR Autobreakers Ltd | Scrap yard | 79 First Drove | Mon–Fri 08:00–17:00 |
| Assured Screening - Peterborough Testing Centre | Medical laboratory | Culley Ct | Daily 08:00–20:00 |
| Athena Holistics | Therapists | 34 Peterborough Rd | Mon–Fri 09:00–20:00 |
| Authentic Acupuncture, Tuina & Herbs | Acupuncturist | Unit 17, Orton Enterprise Centre, Bakewell Rd | Mon 10:00–17:00 |
| Auto Transform | Valeting service | 2 Edmund Dr | Mon–Fri 09:30–17:00 |
| Automobile Locksmith Ltd | Locksmith | 6A Foxcovert Rd | Mon 09:00–17:00 |
| AVS Fencing, Timber & Landscaping Supplies | Fence supply shop | Saville Rd | Mon–Fri 06:30–17:00 |
| B & P Fine Foods (Food Service, Frozen Food, Ice Cream, Ambient, and Drink Wholesaler) | Catering food and drink supplier | 19 Stapledon Rd | Mon–Fri 08:30–17:00 |
| Banjaraa | Mobile caterer | 58 The Causeway | Fri–Sat 10:00–16:00 |
| Barefoot Birth Pools | Maternity shop | 6, Lovells Court, High Causeway | Daily 09:00–17:00 |
| Bargain Buys | Discount shop | Unit 24, The Rivergate Shopping Centre | Mon–Sat 09:00–18:00 |
| Beagle Street Life Insurance | Life insurance agency | Ground Floor, Nene Hall, Lynch Wood | Mon–Fri 08:00–20:00 |
| BEAM Baking Systems Ltd | Wholesaler | Barnack Business Park | Mon–Fri 08:00–17:00 |
| Belfry Facade Systems | Metal fabricator | Unit 8, 11 Business Park, Shrewsbury Ave | Mon 09:00–17:00 |
| Bell Stone Associates Ltd | Design consultant | 6, Viking Trade Park, Newark Rd | Mon–Fri 08:30–17:00 |
| BeMoto Motorbike Insurance | Insurance broker | Britannic House, 11-13 Cowgate | Mon–Fri 09:00–18:00 |
| Bentley Nursing Supplies | Mobility equipment supplier | Unit 2 Benedict Square | Mon 09:00–17:00 |
| Betty Crowson Permanent Make Up Peterborough UK | Permanent make-up clinic | 3 Redwood | Mon–Fri 09:00–18:00 |
| BigBoyclean | Carpet cleaning service | 39 Bardney | Thu–Fri 06:30–22:00 |
| Bijou | Cocktail bar | 51 Bridge St | Wed–Thu 11:00–20:00 |
| Bloods4you - Private & Confidential Blood or Urine Test Service. | Blood testing service | 10 Beckingham | Mon 07:30–22:30 |
| Blue Diamond Coach | Coaching center | Peterborough United Football Club, London Rd | Mon–Fri 09:00–17:30 |
| Body & Soul Therapy | Reiki therapist | 8 Beaulieu Ct | Mon 11:00–15:00 |
| Boheme | Vintage clothing shop | Drayton House, 20 London Rd | Tue 11:30–16:30 |
| Boroughbury Medical Centre | Doctor | Craig St | Thu 08:00–18:30 |
| Boxing Peterborough | Boxing club | 23 Benedict Square | Mon 09:00–12:00 |
| Branded gazebos | Gazebo builder | 38-39 Manasty Rd | Mon–Fri 08:30–17:30 |
| Bretton Medical Practice | Public medical centre | Bretton Health Centre, Rightwell E | Mon–Fri 08:00–18:30 |
| Burch Studio Ltd | Web designer | St Mary's Ave | Mon–Fri 08:00–18:00 |
| BusinessWatch Fire & Security Ltd | Fire alarm supplier | The Metro Centre, 29 Welbeck Way | Thu–Fri Open 24 hours |