| Duo GB Ltd | Exhibition and trade centre | 4 Monks Pond St | Mon–Fri 08:30–17:30 |
| Dust Away Cleaning | Carpet cleaning service | 46 West Ridge | Mon–Sat 09:00–20:00 |
| Duston Garage | Mechanic | 7 Peveril Rd | Mon–Fri 08:00–18:00 |
| E A Agutter Ltd | Painter and decorator | 6 Mallard Cl | Mon–Fri Open 24 hours |
| E.Lee and Sons Butchers | Butchers | 25 The Square | Mon–Thu 08:00–17:30 |
| Ear to Ear Microsuction | Clinic | Building 86, Weedon Ordnance Depot | Mon–Sat 09:00–17:30 |
| Ear Wax Clinic | Audiologist | Ste 6, Spencer House, 3 Spencer Parade | Daily 07:30–21:00 |
| Earthlets | Baby shop | 10, 12 Salthouse Rd | Daily Open 24 hours |
| Earwaxspecialists | Hearing aid shop | Northampton clinic, 72 Abington Ave | Daily 08:00–20:00 |
| EAS Mechanical Limited | Apprenticeship center | 69 St James' Mill Rd | Mon 09:00–16:00 |
| East Lodge Farm Riding Centre | Horse riding school | East Lodge Farm, Washbrook La | Tue–Thu 10:00–21:00 |
| Eat Sleep Bake Repeat | Cooking class | 36 Summerfields | Mon 10:00–10:45, 11:30–12:15, 13:30–14:15, 16:30–17:15 |
| edSAFETY Ltd | Occupational safety and health | The Old Bank, 21 Market Pl | Mon 09:00–17:00 |
| EE Motors | Chauffeur service | 5 Monks Pond St | Mon–Sat 09:00–18:00 |
| Eggfree Cake Inn Distribution Centre | Wholesale bakery | 12 Gatelodge Cl | Mon–Fri 09:30–17:00 |
| EJK Consultancy | IT support and services | Bechtle House, Oxwich Cl | Mon–Fri 09:00–17:30 |
| Elegance Media | Wedding photographer | Ste 3 20, 30A Abington St | Mon 18:00–21:00 |
| Elevations Exhibition and Design Limited | Event planning service | Airport Business Park, Unit 39 Brooklands Gate, Sywell, Wellingborough Rd | Mon–Fri 09:00–17:00 |
| Elite Caravans | Caravan dealer | JBJ Business Park, 36 Northampton Rd | Mon–Sat 09:00–18:00 |
| Elliott's Castles & Ice Creams Ltd | Bouncy castle hire | 18 Frosty Hollow | Daily 07:00–22:00 |
| Emma Helliwell Advanced Aesthetics | Skin care clinic | 19 Church St | Mon 09:00–15:00 |
| EMW Law LLP | Lawyer | 8 Spencer Parade | Thu–Fri 09:00–17:30 |
| Engineering Technoloogies & Manufacturing Ltd | Precision engineer | Longthorne House, 1 Millbrook Cl | Mon–Thu 08:00–16:30 |
| Epi-No UK Ltd | Maternity shop | 22, 24 Harborough Rd | Mon–Sat 09:30–17:00 |
| EPS Trenchless Installations Ltd | Drilling contractor | Mere Farm Business Complex, EPS Poldermere, Red House Ln | Fri 07:00–17:30 |
| Ergonomic Designs Bathrooms | Bathroom supply shop | Cirrus Park Ind Est, unit 2 cirrus Park, Lower Farm Rd | Mon–Fri 09:00–17:00 |
| European Christian Mission | Religious organisation | Moulton Park Business Centre, 34/35 Red House Rd | Mon–Fri 09:00–17:00 |
| Evolve Healthcare Products | Medical equipment supplier | Unit H3, A, Kingsfield Way | Thu 08:30–17:00 |
| Extensions by Debbi | Hair extension technician | 21 Foxford Cl | Mon–Fri 10:30–19:00 |
| Extra Help | Home care service | Kingsthorpe Rd | Mon–Fri 09:00–17:30 |