| East Yorkshire Forklifts | Forklift dealer | Unit 3, East Side Northumberland Avenue | Mon–Fri 08:00–17:00 |
| EastCoast Linemarking | Line marking service | 17 Grosmont Cl | Mon 07:00–17:00 |
| Ecoframes & Energy Saving Solutions Ltd | Window installation service | Unit4, Chapman Street Industrial Estate, Chapman St | Mon–Thu 07:30–16:30 |
| Electric Security Gates Yorkshire | Security system installation service | Unit 5, St Johns Business Park, St John's Grove | Daily Open 24 hours |
| Elliegantly Made | Wedding cake shop | 30 Bond St | Mon–Tue 09:00–17:00 |
| Elliott's Online | Cleaning products supplier | Unit 1, Sidings Business Park, Freightliner Rd | Mon–Thu 07:15–16:15 |
| EMBE Acupuncture and Complementary Health Clinic (Hull & East Yorkshire) | Acupuncturist | 94 Goulton St | Mon 09:00–19:00 |
| Energy Options Ltd | Solar photovoltaic power plant | The Old School Building, Churchill St | Mon–Fri 08:00–16:30 |
| Enviro AC | Property surveyor | 5 Grammar School Yard | Mon–Fri 09:00–17:00 |
| Envirowarm Services Ltd | Central heating service | 7 Sussex Cl | Mon 11:00–14:00 |
| Especially Roofing | Roofing service | Especially Roofing, 66 Bond St | Daily Open 24 hours |
| Esquire Hull | Hairdresser | 900 Spring Bank W | Mon 09:00–14:30 |
| EV Chargers UK | Electric vehicle charging station contractor | C/O Head-On Renewables Limited Teps Yard, Freightliner Rd | Mon–Thu 09:00–16:30 |
| Expotrak Ltd | Demolition contractor | The Station, Station Rd | Mon–Thu 08:00–17:00 |
| EYG Home Improvements | Double glazing installer | Wiltshire Rd | Mon–Fri 09:00–17:30 |
| F&T Vending Co (Falkingham & Taylor Vending) | Vending machine supplier | 40-50 New Cleveland St | Thu–Fri 09:00–17:00 |
| Fastdentuk | Dentist | 59 Priory Grove | Daily Open 24 hours |
| FASTSIGNS Hull | Sign shop | 6 Harpings Rd | Mon–Fri 08:00–16:30 |
| Feel Good Health Club Hull | Gym | Feel Good Health Club, Grange Park Ln | Mon–Thu 06:30–21:00 |
| Fennel UK | Caravan supply shop | Sutton Fields Industrial Estate, Rotterdam Rd | Mon–Thu 08:30–16:30 |
| Finesse Digital | Graphic designer | 31-38 Queen St | Mon 09:00–17:00 |
| firstScan & Window to the Womb Hull | Medical diagnostic imaging centre | Unit 1A, Fretwells Business Park, 65 Oslo Rd | Mon 15:00–19:30 |
| Fisadco Engineering (1980) Ltd | Precision engineer | 55 Raywell St | Mon–Thu 08:00–17:00 |
| Fletchers | Bakery, Deli & Sandwiches | Sandwich shop | 245 Greenwood Ave | Mon–Fri 08:00–16:30 |
| Flex Health - Hull | Physiotherapist | Shed A K3, 200 Clough Rd | Thu 07:00–20:00 |
| Flourish Psychological Therapies | Psychologist | Willerby Square | Mon 10:00–20:00 |
| Ford Transit Centre Hull | Used truck dealer | Priory Park | Mon–Fri 08:00–18:00 |
| Forward Contracting UK Ltd | Shopfitter | Unit 4, Courtney Commercial Business Park, Courtney St | Mon–Fri 09:00–17:00 |
| Fresh Sales & Lettings Limited | Property rental agency | W Dock St | Mon 09:00–17:00 |
| Fuller Health and Medical | Medical centre | 159 Princes Ave | Thu 09:00–17:00 |