| Anthony Meadows Antique furniture restoration. | Antique furniture restoration service | 11 Court Cl | Mon–Fri 08:00–17:30 |
| Apple Tree Montessori Nursery School | Nursery school | The Village Hall, Park Rd | Mon 08:30–15:00 |
| April March Jewellery | Goldsmith | 35A Vale Rd | Mon–Fri 09:00–17:00 |
| Arcadia Cigars - Bond Street | Cigar shop | 6 Bond St | Mon–Fri 10:00–18:00 |
| Archaeology South-East | Heritage preservation | 2 Chapel Pl | Mon–Fri 08:00–17:00 |
| Ardingly Court Surgery | Doctor | 1 Ardingly St | Mon–Fri 08:00–13:00, 14:00–18:00 |
| Armed Forces Careers Office - Brighton | Military recruiting office | 120-121 Queens Rd | Mon–Fri 09:00–17:30 |
| Arthur Denyer Funeral Directors | Funeral home | 18-19 Lewes Rd | Daily Open 24 hours |
| Artist Anon® Limited | Designer clothing shop | 48-51, Kings Road Arches | Thu 11:00–17:00 |
| Artistic MetalWorks | Steel fabricator | Unit 1 Freemans Rd | Mon–Fri 08:30–17:00 |
| Artpod | Arts organisation | 20-22 High St | Wed 10:00–17:00 |
| Aspen Brodie Physiotherapy | Physiotherapist | 111 Cres Dr N | Mon–Wed 09:00–17:00 |
| Atom Graphics Animation | Animation studio | Fl 5 & 6, Tower Point, 44 North Rd | Mon–Fri 09:00–18:00 |
| atomos | Investment service | Ground Floor North, 126 Dyke Rd | Mon–Fri 09:00–17:00 |
| Attenborough Centre for the Creative Arts | Art centre | Gardner Center Rd | Mon–Fri 10:00–16:00 |
| Attuned Therapy | EMDR psychotherapist | 22 Hollingbury Rd | Mon 10:00–18:30 |
| Autohub Autocentres (Brighton) ltd | Vehicle repair shop | UNIT B8-B11, ENTERPRISE ESTATE, Crowhurst Rd | Mon–Fri 08:00–17:00 |
| Autovets Brighton - German, and Sports Car Specialists | Mechanic | 536-540 Falmer Rd | Mon–Fri 08:00–17:00 |
| Ayurveda Brighton | Ayurvedic clinic | 123 Cuckmere Way | Mon–Tue 09:00–17:00 |
| Ayuseva | Alternative medicine practitioner | 23 Green Ridge | Mon–Fri 09:00–17:00 |
| B Company 3PWRR | Military base | 198 Dyke Rd | Mon–Tue 09:00–17:00 |
| Baby Bao | Taiwanese restaurant | The Pond, 49 Gloucester Rd | Mon–Fri 12:00–15:00, 17:00–21:00 |
| Badger Clothing Co Ltd | Men's clothes shop | 26 Bond St | Thu 09:30–18:00 |
| Badgers Tennis Club | Tennis club | Church Pl | Mon–Fri 09:00–18:00 |
| Bagelman | Bagel shop | 7 Bond St | Mon–Fri 09:00–16:00 |
| Bagelman | Bagel shop | 108 St James's St | Thu 08:00–16:00 |
| Bakers, Co-op Funeralcare | Funeral home | 60 Church Rd | Daily Open 24 hours |
| Ball Park BTN | Sports complex | Unit 1, Upperboardwalk | Thu 10:00–23:00 |
| Balmonds Skincare Ltd | Cosmetic products manufacturer | Unit 7, Westergate Business Centre, Westergate Rd | Mon–Fri 09:00–17:00 |
| Baroque Bespoke Jewellery | Jewellery designer | 9-10 Union St | Mon–Sat 10:30–17:00 |