| Electronic Reading Systems Ltd | Computer hardware manufacturer | Wolseley Business Park, 13 Railton Rd | Mon–Fri 09:00–17:30 |
| Eliminate & Elevate | Health consultant | Rm 6, 2 Goldington Rd | Mon–Sat 08:00–21:00 |
| Elite Ground Support Services Ltd | Construction equipment supplier | White House Farm, Begwary Ln | Mon–Fri 07:30–17:00 |
| Elite Skills and Fitness, EliteSF | Children's camp | Hillgrounds Rd | Mon 08:00–18:00 |
| Elite Timber Supplies Ltd | Timber merchant | White House Farm, Begwary Ln | Mon–Fri 07:30–17:30 |
| Emhouse | Hamburger restaurant | 38 The Broadway | Tue–Sun 11:00–23:00 |
| Endometriosis Care | Gynecologist | Bridge House, Britannia Rd | Mon 14:00–18:00 |
| Ents Av Ltd | Audio visual equipment hire service | Stratton Way, 1 Stratton Way | Mon–Fri 09:00–17:00 |
| Equinet Media Ltd | Marketing agency | 1 Goldington Rd | Tue–Wed 09:00–17:30 |
| Excel Accountants | Chartered accountant | Excel House, 3 Duke St | Mon–Fri 09:00–17:00 |
| Executive Physiotherapy Ltd | Physical therapy clinic | 7 Westfield Rd | Mon–Fri 09:00–16:00 |
| Expeditors International (UK) LTD | Logistics service | Unit 5 Swallow Way | Mon–Fri Open 24 hours |
| Extreme Sports Therapy | Sports injury clinic | Unit 1, Swan Works, Box End Rd | Mon–Fri 09:00–20:00 |
| Fabric World | Fabric shop | 3 Thurlow St | Mon–Sat 09:30–16:30 |
| Face-T | Tailor | Unit 23a, The Old Water Mill, Barton Mill Lane, Faldo Rd | Mon 09:30–17:30 |
| Fade Audio Visual | Home automation company | 8 Devon Rd | Mon 09:00–17:00 |
| Farrow Civil Engineering Ltd | Civil engineering | 7 Mill Ln | Mon–Tue 08:30–17:30 |
| Firewoodshire | Firewood supplier | College Farm, Lovell Rd | Fri 09:00–18:00 |
| Flitwick and Ampthill Bathrooms | Bathroom supply shop | Unit 6, 12 Windmill Rd | Mon–Fri 09:00–17:00 |
| Flitwick Community Football Centre | Football club | Flitwick Football Centre, Ampthill Rd | Sat 08:30–18:00 |
| Flitwick Motorcycles | Motor scooter dealer | Station Rd | Tue 08:30–18:00 |
| Flitwick Mowers Ltd | Lawn mower shop | 7 Lyall Court | Tue–Thu 09:00–17:00 |
| Flying Horse Clophill | Gastropub | Bedfordshire, 2 The Green | Mon–Thu 11:00–23:00 |
| Fordfield Nursery School | Nursery school | Fordfield House, Fordfield Rd | Mon–Fri 08:00–18:00 |
| Forever Health Studios | Fitness centre | Unit 2, 4 Wolseley Rd | Mon 07:00–19:00 |
| Forte Motors Limited | MOT centre | 46-48 Stuart Rd | Mon–Fri 08:30–17:00 |
| FruehaufParts | Truck parts supplier | Newton Trailers Ltd, Station Rd | Mon–Fri 08:00–17:00 |
| G Moore Haulage Ltd | Waste-management service | Manor Rd | Mon–Fri 07:00–17:30 |
| G.S. Chaggar Engineering | Sheet metal contractor | Murdock Road Manton Industrial Estate, Murdock Rd | Mon–Fri 08:30–17:00 |
| Gallone's Parlour | Ice cream shop | 1, 2 The Arcade, High St | Mon–Sat 09:30–17:00 |