| Complete Fabrication - 3D Printing Prototyping Specialist | 3D printing service | Unit 4b, LION WORKS, Station Rd E | Mon–Fri 08:30–17:30 |
| K L Giddings Ltd | Engineer | LION WORKS, Station Rd E | Mon–Fri 06:00–18:00 |
| T D Kent Sunglasses Manufacturer | Sunglasses shop | Unit 4b, Lion Works, Station Rd E | Mon 09:00–17:30 |
| Whittlesford Memorial Hall | Village hall | Mill Ln | Mon–Fri 09:00–23:00 |
| Village Vet Whittlesford | Veterinarian | 39 Station Rd W | Daily 08:30–19:00 |
| Business Quotes | Business-to-business service | 1 W End | Daily Open 24 hours |
| National Highways Depot | Road construction company | 57 Station Rd E | Mon–Sat 08:00–17:00 |
| Shandrum Office Supplies Ltd | Office supplies shop | Unit 3, Lion Works, Station Rd E | Mon 09:00–17:00 |
| Wells Park Caravan Storage | Storage facility | Wells Park Farm, 3 Shelford Rd | Mon 08:00–17:00 |
| Whittlesford's Tiney Home Nursery | Childminder | 32 Mill Ln | Wed 08:00–17:30 |
| Cambridge Money Coaching | Coaching center | Moor House, Whippletree Rd | — |