| akarmalife studios | Yoga studio | 2nd Floor, 4A Church St | Mon–Fri 07:00–21:30 |
| Ampthill Antiques Emporium | Antique shop | Courtyard at rear of, 6 Bedford St | Mon 10:00–17:00 |
| Ampthill Banking Hub | Bank | 8, 10 Church St | Mon–Fri 09:00–17:00 |
| Ampthill Library | Library | 1 Dunstable St | Tue 09:00–13:00 |
| Ampthill Motors | Mechanic | Unit A, L W Vass Ltd, Ampthill Industrial Estate Station Rd | Mon–Fri 08:30–17:30 |
| Ampthill Sushi Daily | Sushi takeaway | 21-25 Bedford St | Mon–Tue 08:00–20:30 |
| Cakestand and Crumb | Tea room | 7 Kings Arms Yard | Tue–Sat 10:00–16:30 |
| Cambridge Wine Merchants Ampthill | Wine shop | 12 Church St | Tue 11:00–19:00 |
| Heritage Park Family Law | Family lawyer | 3D Woburn St | Mon–Fri 09:00–18:00 |
| Houghton House | Historical landmark | Houghton House, Hazelwood Ln | Daily 10:00–16:00 |
| Italia Stone | Stone cutter | Unit 8, Ampthill Business Park Station Rd | Mon–Thu 09:00–13:00, 14:00–17:00 |
| Jodie Gearing Jewellery Design | Jewellery designer | 157 Oliver St | Tue 10:00–17:00 |
| MDG Builders | Deck builder | 17 Coltsfoot Cor | Mon–Thu 08:00–17:30 |
| Meads Racing | Motorcycle shop | Unit B Station Rd | Mon–Fri 09:00–17:00 |
| Oak Fostering | Childcare agency | Ste 1, The Limes, 12 Dunstable St | Mon–Fri 09:00–17:00 |
| Patternmatch Interiors | Blinds shop | 106A Dunstable St | Mon–Tue 10:00–16:00 |
| Perceptive Financial Solutions Ltd | Financial planner | 7 Woburn St | Mon–Fri 09:00–17:00 |
| Processworx | Marketing agency | 59 Willow Wy. | Mon–Fri 08:00–16:00 |
| Steph Hammond - Pilates & Wellness | Pilates studio | Unit 4, Ampthill Business Park Station Rd | Mon 09:00–12:00, 17:00–20:30 |
| The Nine Ball Tattoo Studio | Tattoo and piercing shop | 2 Church St | Mon 09:30–19:00 |
| Woodstone Bathrooms | Bathroom renovator | 15 Bedford St | Mon–Fri 10:00–17:00 |
| Big 10 - Smoke & Grill | Bar | 4-8 Church St | Wed–Thu 17:00–23:00 |
| Silver Lining Tattoo Ampthill | Tattoo shop | 3-5 Church St | Tue–Wed 10:00–18:00 |
| Ampthill Baptist Church | Baptist church | 48 Dunstable St | Sun 10:45–12:00, 18:30–20:00 |
| Ampthill Fireplaces | Fireplace shop | 1 Bedford St | Mon 10:00–17:00 |
| Ampthill Glass Centre Ltd | Glass repair service | Ampthill Business Park, Ampthill Business Park Station Rd | Fri 09:00–13:00, 14:00–17:00 |
| Ampthill Great Park West Car Park | Free car park | Woburn Rd | Fri–Sat Open 24 hours |
| BES Geomatics Ltd | Land surveyor | The Loft, 111 Dunstable St | Mon–Fri 08:30–18:00 |
| Handling Equipment Sales Ltd | Material handling equipment supplier | Unit 2, Ampthill Business Park Station Rd | Mon–Fri 08:30–17:00 |
| Ink Print Solutions Ltd - Commercial Printers Bedfordshire | Printing shop | The Loft, Barn Studios, 3 Church St | Mon–Fri 09:00–17:00 |